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The possible involvement of nitric oxide (NO) in the pathogenesis of arthritis, using N-nitrosopiperidine (NSP) as a model compound, was investigated using the rat’s hind paw edema model and the spontaneous arthritis in DBA/1J mice model. Intra-articular administration of NSP produced a dose-dependent increase in the paw volume, which suggested the participation of NO. The contractile activity of the isolated isolated gastrocnemius muscle was depressed by the co-administration of NSP with N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES). This suggests the existence of a free radical which in turn caused direct contraction of the muscle through the action of NO. In DBA/1J mice, a drug which inhibited the production of NO from lipopolysaccharide (LPS) induced arthritis also suppressed the arthritic activity of LPS
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Reich, N., Spinnler, B., Holzmann, M., Bühlmann, P. (2011). A structural equation modeling approach to detecting alterations in edge dynamics in co-authorship networks. *PLoS ONE* 6(6): e23121. doi:10.1371/journal.pone.0023121.
Reichardt, J., Forster, A., Smith, A.J. (2008). Measuring and detecting simulated and real scale-free network patterns in collaboration networks. *Phys. Rev. Lett.* 101: 218701. doi:10.1103/PhysRevLett.101.218700.
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Raftery, A.E. (1995). A direct approach to posterior distributions for Bayesian model choice. *Ann. Statist.* 23:895-904.
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A SV40-transformed cell line of human fibroblasts (Küter-1) is introduced in this report. The cells grew in suspension, divided on the third day post-establishment, and formed colonies on the fifth day post-cultivation. The clonal cultures were uniform in shape and growth characteristics, and were highly heterogeneous for their oncogenicity, in that some cells formed tumours in severe combined immunodeficient mice and other did not. The chromosomal analysis demonstrated that the cell lines contained the full complement of chromosomes, and some of them appeared to be diploid. The transient expression analysis of a specific transgene demonstrated the characteristics of SV40-transformed cells. These results showed that Küter-1 cells are highly homogeneous and reliable in vitro model cells.Stand up for Roe
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